Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2472 |
| PDBID: | 6MI2 |
| Chains: | AB_C |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.72 |
| Reference: | 10.1038/s41467-018-07136-7 |
| Antibody | |
| Antibody: | Utomilumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Utomilumab(Phase-II) |
| Antigen | |
| Antigen: | Tumor necrosis factor receptor superfamily member 9 (CD137) |
| Antigen mutation: | No |
| Durg Target: | Q07011 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6MI2_A|Chain A, C[auth D]|Utomilumab Fab heavy chain|Homo sapiens (9606) EVQLVQSGAEVKKPGESLRISCKGSGYSFSTYWISWVRQMPGKGLEWMGKIYPGDSYTNYSPSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARGYGIFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAAAHHHHHHHH |
Light Chain: B
Mutation: NULL
| >6MI2_B|Chain B, D[auth E]|Utomilumab Fab lambda chain|Homo sapiens (9606) SYELTQPPSVSVSPGQTASITCSGDNIGDQYAHWYQQKPGQSPVLVIYQDKNRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCATYTGFGSLAVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: C
Mutation: NULL
| >6MI2_C|Chain E[auth C], F|Tumor necrosis factor receptor superfamily member 9|Homo sapiens (9606) QDPCSNCPAGTFCDNNRNQICSPCPPNSFSSAGGQRTCDICRQCKGVFRTRKECSSTSNAECDCTPGFHCLGAGCSMCEQDCKQGQELTKKGCKDCCFGTFNDQKRGICRPWTNCSLDGKSVLVNGTKERDVVCGPSPENLYFQG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR31 TYR32 TRP33 LYS50 TYR52 ASP55 TYR57 ARG98 TYR100 ILE102 ASP104 TYR105 B: GLY28 ASP29 GLN30 TYR31 LEU45 TYR48 GLN49 ASP50 ARG53 PRO54 SER55 ASN65 TYR90 GLY92 SER95 C: ARG66 VAL71 PHE72 CYS94 LEU95 GLY96 ALA97 GLY98 SER100 MET101 CYS102 GLU103 GLN104 GLU111 LEU112 THR113 LYS114 LYS115 PHE125 ASP127 GLN128 ARG130 GLY131 ILE132 ARG134 PRO135 THR137 ASP155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
