Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2454 |
| PDBID: | 6M58 |
| Chains: | CD_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.95 |
| Reference: | 10.1016/j.bbrc.2020.03.133 |
| Antibody | |
| Antibody: | SL335 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Serum albumin |
| Antigen mutation: | No |
| Durg Target: | P02768 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6M58_C|Chain B[auth C], E[auth H]|Heavy chain of the SL335 antibody fab|Homo sapiens (9606) QVQLVQSGGGPVKPGGSLRLSCAASGFMFRAYSMNWVRQAPGKGLEWVSSISSSGRYIHYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCARETVMAGKALDYWGQGTLVTVSSASTKGPSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSS |
Light Chain: D
Mutation: NULL
| >6M58_D|Chain C[auth D], F[auth L]|Light chain of the SL335 antibody Fab|Homo sapiens (9606) DIVLTQSPGTLSLSPGETATLSCRASQSVGSNLAWYQQKPGQAPRLLIYGASTGATGVPARFSGSRSGTDFTLTITSLQPEDFATYYCQQYYSFLAKTFGQGTQLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSNTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGES |
Antigen
Chain: B
Mutation: NULL
| >6M58_B|Chain A, D[auth B]|Serum albumin|Homo sapiens (9606) DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLY26 PHE27 MET28 ARG30 ALA31 TYR32 SER33 SER52 SER53 ARG55 TYR56 GLU95 THR96 VAL97 MET98 ALA99 D: ILE2 SER28 GLY30 SER31 ASN32 TYR49 ARG66 SER67 GLY68 TYR91 TYR92 SER93 PHE94 B: HIS9 LYS212 LYS225 GLU227 PHE228 ALA229 GLU230 SER232 LYS233 THR236 ASP256 ASP259 LYS262 TYR263 GLU266 ASN267 ASP269 SER270 PRO299 ASP308 PHE309 ASN318 GLU321 ALA322 ASP324 VAL325 PHE326 MET329 GLU333 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
