Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2442 |
| PDBID: | 6LZ9 |
| Chains: | HL_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | t8E4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatocyte growth factor (HGF) |
| Antigen mutation: | No |
| Durg Target: | P14210 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6LZ9_H|Chain C[auth H]|Heavy chain of t8E4 Fab fragment|Mus musculus (10090) QVQLKESGPDLVQPSQTLSLTCTVSGFSLTGYGVHWVRQPPGKGLEWVGTLGWNDKKYYNSALKSRLSISRDTSKNQVFLKLSSLETEDTAMYYCTRDGGLLFAYYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >6LZ9_L|Chain D[auth L]|Light chain of t8E4 Fab fragment|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTFSCRASQDISNYLNWYQQKPDGTVKLLIFYTSRLHSGVPSRFSGSGSGTDYSLTIANLEQEDFATYFCQQDSKHPFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >6LZ9_B|Chain B|Hepatocyte growth factor|Homo sapiens (9606) VVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKSKQVLQVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLQESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQSRLENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER25 GLY26 PHE27 SER28 THR30 GLY31 TYR32 TRP53 ASN54 ASN76 GLY97 LEU98 LEU99 L: TYR32 PHE49 TYR50 ARG53 LEU54 SER56 ASP91 SER92 B: ASN497 GLY498 ILE499 PRO500 THR501 ARG502 THR503 ASN504 ILE505 GLY506 TRP507 ASP592 PHE593 SER595 SER611 CYS612 SER613 TYR615 LEU628 ARG630 VAL631 HIS633 TYR635 ILE664 VAL678 CYS679 GLU680 LYS683 ARG685 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
