Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2441 |
| PDBID: | 6LZ2 |
| Chains: | B_A |
| Organism: | Galaxea fascicularis, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.03 |
| Reference: | 10.1038/s42003-020-01478-z |
| Antibody | |
| Antibody: | 44 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Thermostable green fluorescent protein (TGP) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6LZ2_B|Chain B, D|synthetic nanobody (sybody) 44 against the thermostable green fluorescent protein (TGP)|Lama glama (9844) GSSSQVQLVESGGGLVQAGGSLRLSCAASGFPVGRASMWWYRQAPGKEREWVAAISSYGWVTAYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCEVSVGTGYRGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >6LZ2_A|Chain A, C|Thermostable green fluorescent protein|Galaxea fascicularis (46745) MASVIKPEMKIKLRMEGAVNGHKFVIEGEGIGKPYEGTQTLDLTVEEGAPLPFSYDILTPAFQYGNRAFTKYPEDIPDYFKQAFPEGYSWERSMTYEDQGICIATSDITMEGDCFFYEIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVEMALLLEGGGHYRCDFKTTYKAKKDVRLPDAHEVDHRIEILSHDKDYNKVRLYEHAEARYSGGGSGGGHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG31 ALA32 SER33 TRP35 TYR37 ARG45 TRP47 ALA50 ILE51 SER52 SER53 TYR54 TRP56 VAL57 THR58 ALA59 TYR60 ALA61 ASP62 LYS65 SER99 VAL100 GLY101 THR102 A: ALA18 GLY21 LYS23 SER89 GLU91 ARG92 SER93 THR95 GLN99 ILE101 ILE103 THR105 ASP107 GLU118 ARG120 ASP122 GLY123 THR124 ASN125 LYS175 THR177 LYS179 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
