Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2435 |
| PDBID: | 6LXK |
| Chains: | HL_A |
| Organism: | Influenza A virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.61 |
| Reference: | 10.1128/mBio.02315-20 |
| Antibody | |
| Antibody: | Z2B3 Fab |
| Antibody mutation: | Yes |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | H1N1 neuraminidase |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: D102R
| >6LXK_H|Chain B[auth E], G[auth H], I[auth J]|Heavy chain of Z2B3-D102R Fab|Homo sapiens (9606) EVQLVQSGAEVKKPGSSVKVSCKASGGPFSSYGFSWVRQAPGQGLEWMGGIIPVYGTTNYAQKFQGRVTITADESTSTAYMELSSLKSEDTAVYYCARDLQRTPMVDRIIGSYYYYNGLDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >6LXK_L|Chain C[auth F], H[auth K], J[auth L]|Light chain of Z2B3-D102R Fab|Homo sapiens (9606) QSVLTQPASVSGSPGQSITISCTGASSDVGDYNYVSWYQQHPGKAPKLMIYDVSNRPSGVSNRFSGSKSGDTASLTISGLQAEDEADYYCSSYTRSSSVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >6LXK_A|Chain A, D[auth B], E[auth C], F[auth D]|Neuraminidase|Influenza A virus (11320) MNPNQKIITIGSVCMTIGMANLILQIGNIISIWVSHSIQLGNQSQIGTCNQSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIYSKDNSVRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIKDRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDSGAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTIMTDGPSDGQASYKIFRIEKGKIVKSVEMNAPNYHYEECSCYPDSSEITCVCRDNWHGSNRPWVSFNQNLEYQIGYICSGVFGDNPRPNDKTGSCGPVSSNGANGVKGFSFKYGNGVWIGRTKSISSRKGFEMIWDPNGWTGTDNKFSIKQDIVGINEWSGYSGSFVQHPELTGLDCIRPCFWVELIRGRPEENTIWTSGSSISFCGVNSDTVGWSWPDGAELPFTIDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 SER31 VAL54 TYR55 GLY56 GLU74 ARG102 PRO104 MET105 VAL106 ASP107 ARG108 ILE109 ILE110 GLY111 TYR113 L: GLY30 ASP31 TYR32 TYR34 TYR93 ARG95 A: ARG118 GLU119 ILE149 LYS150 ASP151 ARG152 TRP179 SER180 ASP199 ASN222 ILE223 ARG225 GLU228 PRO246 SER247 ASP248 GLY249 GLN250 ASN273 GLU277 GLU278 ARG293 ASN295 TRP296 HIS297 SER339 ASN341 GLY342 ALA343 ASN344 GLY345 SER367 ARG368 TYR402 ILE427 ARG430 PRO431 GLU432 THR438 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
