Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2428 |
| PDBID: | 6LR7 |
| Chains: | B_A |
| Organism: | Aequorea victoria, Camelus bactrianus |
| Method: | XRD |
| Resolution (Å): | 1.67 |
| Reference: | 10.1038/s41598-020-62606-7 |
| Antibody | |
| Antibody: | LaG16 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Green fluorescient protein uv |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6LR7_B|Chain B|Nanobody LaG16|Camelus bactrianus (9837) GPHMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKNTVDLQMDNLEPEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVS |
Antigen
Chain: A
Mutation: NULL
| >6LR7_A|Chain A|Green fluorescent protein|Aequorea victoria (6100) GPHMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLLKDPNEKRDHMVLLEFVTAAGITHGMDELYK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG28 PHE30 SER31 THR32 SER33 THR53 TRP54 THR55 VAL56 ASN58 ARG75 ARG100 SER101 ARG102 GLY103 TYR104 VAL105 LEU106 A: LEU7 LEU15 GLU17 ASP19 PRO89 GLU90 ARG109 GLU111 LYS113 PHE114 GLU115 GLY116 ASP117 THR118 VAL120 ARG122 GLU124 ILE188 GLY189 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
