Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2426 |
| PDBID: | 6LHP |
| Chains: | HL_C |
| Organism: | Mus musculus, Coxsackievirus A16 |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1016/j.chom.2020.01.003 |
| Antibody | |
| Antibody: | 14B10 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | coxsackievirus A16 VP3 protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6LHP_H|Chain B[auth H]|heavy chain variable region of Fab 14B10|Mus musculus (10090) QVQLQQSGPELVKPGASVKISCKASGYEFSRSWMNWVKQRPGQGLEWIGRIYPGDGDTNYNGKFRDKATLTADKSSSTAYMQLSRLTSVDSAVYFCARRRGYFDVWGAGTTVTVS |
Light Chain: L
Mutation: NULL
| >6LHP_L|Chain A[auth L]|light chain variable region of Fab 14B10|Mus musculus (10090) DIQMTQSSSYLSVSLGGRVTITCKASDHINNWLAWYQQKPGNAPRLLISGATSLETGIPSRFSGSGSGKDYTLSITSLQTEDVGTYYCQQYWSTPTFGGGTKLEIKR |
Antigen
Chain: C
Mutation: NULL
| >6LHP_C|Chain E[auth C]|VP3 protein|Coxsackievirus A16 (31704) GIPTELKPGTNQFLTTDDGVSAPILPGFHPTPPIHIPGEVHNLLEICRVETILEVNNLKTNETTPMQRLCFPVSVQSKTGELCAAFRADPGRDGPWQSTILGQLCRYYTQWSGSLEVTFMFAGSFMATGKMLIAYTPPGGNVPADRITAMLGTHVIWDFGLQSSVTLVVPWISNTHYRAHARAGYFDYYTTGIITIWYQTNYVVPIGAPTTAYIVALAAAQDNFTMKLCKDTEDIEQTANIQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN30 TRP32 TYR91 TRP92 H: ARG99 ARG100 GLY101 C: VAL75 GLN76 SER77 LYS78 VAL204 THR210 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
