Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2422 |
| PDBID: | 6LBH |
| Chains: | CD_B |
| Organism: | Thermus thermophilus HB8, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1371/journal.pbio.3001231 |
| Antibody | |
| Antibody: | YN0705 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Magnesium transporter MgtE |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6LBH_C|Chain D[auth C], F[auth E]|Fab heavy chain|Mus musculus (10090) EVKLQESGVELVKPGASVKISCKASGYSFTGYNMNWVKQSHGKSLEWIGNISPYYGTSIYNQNFKGKATLTVDRSSSTAYMQLNSLTSEDSAVYYCARGESFSNYEGYYAMDYWGQGTSVIVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: D
Mutation: NULL
| >6LBH_D|Chain C[auth D], E[auth F]|Fab light chain|Mus musculus (10090) DVLMTQTPLSLPVSLGDQASISCRSSQSLVHSDGNTYLHWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: B
Mutation: NULL
| >6LBH_B|Chain A, B|Magnesium transporter MgtE|Thermus thermophilus HB8 (300852) LVYSEAGPVALWLARVRWLVILILTGMVTSSILQGFESVLEAVTALAFYVPVLLGTGGNTGNQSATLIIRALATRDLDLRDWRRVFLKEMGVGLLLGLTLSFLLVGKVYWDGHPLLLPVVGVSLVLIVFFANLVGAMLPFLLRRLGVDPALVSNPLVATLSDVTGLLIYLSVARLLLE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: HIS31 SER32 ASP33 GLY34 TYR37 SER96 THR97 VAL99 TRP101 C: ASN33 ASN50 SER52 TYR54 TYR55 THR57 SER58 ILE59 GLU100 SER101 PHE102 SER103 ASN104 TYR105 TYR109 B: LEU342 ALA343 THR344 ARG345 ASP348 LEU349 ARG350 ASP351 TRP352 ARG353 ARG354 ARG413 ARG414 LEU415 GLY416 VAL417 ASP418 PRO419 LEU421 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
