Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2407 |
| PDBID: | 6KO5 |
| Chains: | HL_A |
| Organism: | Escherichia coli, Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1038/s41467-020-17554-1 |
| Antibody | |
| Antibody: | 7881 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Chimera of Soluble cytochrome b562 and Growth hormone secretagogue receptor type 1 |
| Antigen mutation: | Yes |
| Durg Target: | Q92847; P0ABE7; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6KO5_H|Chain B[auth H]|Fab7881 Heavy Chain|Mus musculus (10090) EVQLQQSGAELVRPGASVKLSCKASGYTFTDYEMHWVKQTPVHGLEWIGAIDPETGGTVYNQKFKGKATLTADISSTTAYMELRSLTSEDSAVYFCISEDIDESKDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: L
Mutation: NULL
| >6KO5_L|Chain C[auth L]|Fab7881 Light Chain|Mus musculus (10090) DIVMTQSPSSLAVSAGEKVTLSCKSSQSLFNSRTRKNYLAWYQQKPGLSPTLLIYWASTRKSGVPDRFTGSGSGTDFTLTITSVQAEDLAVYYCKQSYYLRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: M7W/H102I/R106L/T208K/N266Q
| >6KO5_A|Chain A|Chimera of Soluble cytochrome b562 and Growth hormone secretagogue receptor type 1|Escherichia coli (562) ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYIQKYLSLGDELLQLFPAPLLAGVTATCVALFVVGIAGNLLTMLVVSRFRELRTTTNLYLSSMAFSDLLIFLCMPLDLVRLWQYRPWNFGDLLCKLFQFVSESCTYAKVLTITALSVERYFAICFPLRAKVVVTKGRVKLVIFVIWAVAFCSAGPIFVLVGVEHEQGTDPWDTNECRPTEFAVRSGLLTVMVWVSSIFFFLPVFCLTVLYSLIGRKLWRRRRGDAVVGASLRDQNHKQTVKMLAVVVFAFILCWLPFHVGRYLFSKSFEPGSLEIAQISQYCNLVSFVLFYLSAAINPILYNIMSKKYRVAVFRLLGFEPFSQR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: THR30 ASP31 GLU33 HIS35 TRP47 ALA50 ILE51 ASP52 GLU54 THR55 GLY57 THR58 VAL59 GLU99 ASP100 ILE101 GLU103 LYS105 ASP106 L: TYR38 LYS95 SER97 LEU100 ARG101 A: LYS238 ARG241 ARG242 ARG243 ARG244 GLY245 ASP246 ALA247 VAL248 VAL249 GLY250 SER252 ASP255 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
