Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2395 |
| PDBID: | 6K69 |
| Chains: | A_B |
| Organism: | Homo sapiens, Tequatrovirus T4 |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1073/pnas.1910080116 |
| Antibody | |
| Antibody: | Anti-huntingtin VL-intrabody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein A #9213 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >6K69_A|Chain A|3LRH intrabody|Homo sapiens (9606) MGSSHHHHHHSSGLVPRGSHMGSQPVLTQSPSVSAAPRQRVTISVSGSNSNIGSNTVNWIQQLPGRAPELLMYDDDLLAPGVSDRFSGSRSGTSASLTISGLQSEDEADYYAATWDDSLNGWVFGGGTKVTVLSA |
Antigen
Chain: B
Mutation: NULL
| >6K69_B|Chain B|Engineered T4 lysozyme|Enterobacteria phage T4 (10665) MGSSHHHHHHSSGLVPRGSHMNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPVEGGGGSGGGGSEKLMKAFESLSLFQAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYANL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: PRO5 ASN38 ILE40 GLN42 ALA47 PRO48 GLU49 LEU50 TYR53 ASP54 LEU57 PRO60 TYR91 ALA93 TRP95 TRP102 PHE104 B: TYR25 LYS51 LEU52 LYS54 ALA55 PHE56 GLU57 SER58 LEU59 LEU61 PHE62 GLN63 LYS65 SER66 ASN77 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
