Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2375 |
| PDBID: | 6JMR |
| Chains: | EF_B |
| Organism: | Mus musculus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 4.10 |
| Reference: | 10.1038/s41594-019-0237-7 |
| Antibody | |
| Antibody: | HBJ127 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 4F2 cell-surface antigen heavy chain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6JMR_E|Chain A[auth E]|Antibody|Mus musculus (10090) QVKLLESGPGLVQPSQSLSITCTVSGFSLTTYGIHWVRQPPGKGLEWLGVIWSNGRIDYNAAFISRLSITKDNSKSQVFFKMNSLQDDDTAIYYCARNVYDSLTWFTYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: F
Mutation: NULL
| >6JMR_F|Chain B[auth F]|Antibody|Mus musculus (10090) DIQMTQSPSSLAVSVGEKVTMTCKSSQSLLYSSNQKNYLAWYQQKPGQSPKLLIFWASTRESGVPDRFTGSGSGTDFPLTISSVKAEDLAVYFCQQYTSYPTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: B
Mutation: NULL
| >6JMR_B|Chain C[auth B]|4F2 cell-surface antigen heavy chain|Homo sapiens (9606) GSELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSELGSHCVAQTGLELLASGDPLPSASQNAEMIETGSDCVTQAGLQLLASSDPPALASKNAEVTGTMSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKFTGLSKEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TRP52 ASN54 GLY55 ARG56 ASP58 TYR100 ASP101 SER102 LEU103 F: TYR31 SER32 SER33 ASN34 TYR38 TYR97 THR98 SER99 TYR100 B: ALA476 ALA477 ALA478 LEU479 PRO480 GLY481 GLN482 PRO483 MET484 GLU493 SER494 PHE496 PRO497 ASP498 ILE499 PRO500 GLY501 ALA502 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
