Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2355 |
| PDBID: | 6J11 |
| Chains: | DE_C |
| Organism: | Middle East respiratory syndrome-related coronavirus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/s41467-019-10897-4 |
| Antibody | |
| Antibody: | 7D10 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MERS-CoV spike NTD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >6J11_D|Chain D, F, H|VH of 7D10|Mus musculus (10090) GGGSGGGSGGGSEVQLVESGAEVVKPGASVKMSCKASGYPFTSYNIHWIKQTPGQGLEWIGAIYPGNGDTSYTQKFKVKATLTSDKSSSTAYMQLSSLTSEDSAVYFCARYGNYPSYAMDYWGQGTSVTVSS |
Light Chain: E
Mutation: NULL
| >6J11_E|Chain E, G, I[auth L]|VL of 7D10|Mus musculus (10090) DIVMTQSPASLTVSLGQRATISCRASKSVSASGYNYLHWYQQRPGQPPKLLIYLAFNLESGVPARFNGSGSGTDFTLNIHPVEEEDAATYYCQHSRDLPFTFGSGTKLEIK |
Antigen
Chain: C
Mutation: NULL
| >6J11_C|Chain A, B, C|N-terminal domain of Spike glycoprotein|Middle East respiratory syndrome-related coronavirus (1335626) YVDVGPDSVKSACIEVDIQQTFFDKTWPRPIDVSKADGIIYPQGRTYSNITITYQGLFPYQGDHGDMYVYSAGHATGTTPQKLFVANYSQDVKQFANGFVVRIGAAANSTGTVIISPSTSATIRKIYPAFMLGSSVGNFSDGKMGRFFNHTLVLLPDGCGTLLRAFYCILEPRSGNHCPAGNSYTSFATYHTPATDCSDGNYNRNASLNSFKEYFNLRNCTFMYTYNITEDEILEWFGITQTAQGVHLFSSRYVDLYGGNMFQFATLPVYDTIKYYSIIPHSIRSIQSDRKAWAAFYVYKLQPLTFLLDFSVDGYIRRAIDCGFNDLSQLHCSYES |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER31 TYR32 ASN33 HIS35 ALA50 ILE51 TYR52 ASN55 ASP57 THR58 SER59 TYR60 TYR99 ASN101 TYR102 TYR105 E: ALA56 SER57 TYR59 TYR61 SER120 ARG121 ASP122 LEU123 C: TYR18 VAL19 ASP20 VAL21 GLY22 PRO23 ASP24 SER25 VAL26 LYS27 SER28 ILE186 GLU188 PRO189 ARG190 SER191 THR212 ASN222 ASN226 GLU230 ASN233 LEU234 ARG235 ASN236 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
