Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2341 |
| PDBID: | 6IR1 |
| Chains: | B_A |
| Organism: | Anaplasma marginale, Camelus bactrianus |
| Method: | XRD |
| Resolution (Å): | 1.92 |
| Reference: | 10.1002/pro.4194 |
| Antibody | |
| Antibody: | LaM4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MCherry fluorescent protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6IR1_B|Chain B|mCherry's nanobody LaM4|Camelus bactrianus (9837) AQVQLVESGGSLVQPGGSLRLSCAASGRFAESSSMGWFRQAPGKEREFVAAISWSGGATNYADSAKGRFTLSRDNTKNTVYLQMNSLKPDDTAVYYCAANLGNYISSNQRLYGYWGQGTQVTVSSPFTLE |
Antigen
Chain: A
Mutation: NULL
| >6IR1_A|Chain A|MCherry fluorescent protein|Anaplasma marginale (770) GMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN2 ARG28 PHE29 GLU31 SER32 SER53 TRP54 SER55 ALA58 THR59 ASN60 LEU101 GLY102 ASN103 TYR104 ILE105 SER106 ASN108 LEU111 GLY113 TYR114 A: ALA6 ILE7 ILE8 LYS9 GLU10 TYR38 ALA77 ASP78 ILE79 PRO80 ASP81 LYS84 LEU85 PHE87 LYS185 PRO186 VAL187 GLN188 LEU189 PRO190 GLY191 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
