Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2323 |
| PDBID: | 6IKM |
| Chains: | a_A |
| Organism: | Pseudomonas aeruginosa, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1016/j.jmb.2019.10.026 |
| Antibody | |
| Antibody: | ScFv5 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SpuE-Spermidine Polyamine transport protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: a
Mutation: NULL
| >6IKM_A|Chain A, AA[auth B], C, CA[auth D], E, EA[auth F], G, GA[auth H], I, IA[auth J], K, M[auth L], O[auth M], Q[auth N], S[auth O], U[auth P], W[auth Q], Y[auth R]|Polyamine transport protein|Pseudomonas aeruginosa (287) GPLGSSLHIYNWTDYIAPTTLKDFTKESGIDVSYDVFDSNETLEGKLVSGHSGYDIVVPSNNFLGKQIQAGAFQKLDKSKLPNWKNLDPALLKQLEVSDPGNQYAVPYLWGTNGIGYNVAKVKEVLGDQPIDSWAILFEPENMKKLAKCGVAFMDSGDEMLPAALNYLGLDPNTHDPKDYKKAEEVLTKVRPYVSYFHSSKYISDLANGNICVAFGYSGDVFQAAARAEEAGKGIDIQYVIPKEGANLWFDLMAIPADAKAADNAYAFIDYLLRPEVIAKVSDYVGYANAIPGARPLMDKSVSDSEEVYPPQAVLDKLYVSAVLPAKVLRLQTRTWTRIK |
Antigen
Chain: A
Mutation: NULL
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| a: THR32 SER33 TYR34 GLY35 TRP52 ASN54 ASN56 ASN61 ASP101 LYS102 ARG103 SER159 GLY163 ARG164 ASN165 TYR166 ASP184 ASN185 LYS200 TRP225 SER227 ARG230 VAL232 A: PHE244 GLN260 GLU298 GLY315 ARG317 PRO318 LEU319 MET320 ASP321 LYS322 SER323 SER325 ASP326 SER327 GLU328 GLU329 TYR331 PRO332 PRO333 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
