Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2313 |
| PDBID: | 6IEK |
| Chains: | EF_D |
| Organism: | Rift Valley fever virus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1038/s41564-019-0411-z |
| Antibody | |
| Antibody: | R12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rift Valley fever virus (RVFV) NSmGnGc gp |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6IEK_E|Chain B, E|Heavy chain of Fab R12|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKVSGYTLTELSMHWVRQAPGKGLEWMGGFDREDGETIYAQKFQGRVTMTEDTSTDTAYMELSSLRSEDTAVYYCAIDPIRYNWNYGDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: F
Mutation: NULL
| >6IEK_F|Chain C, F|Light chain of Fab R12|Homo sapiens (9606) LPVLTQPPSSSASPGESARLTCTLPSDISVSSYNIYWYQQKPGSPPRFLLYYYSDSDKGQGSGVPSRFSGSKDASDNTGILLISGLQSEDEADYYCMIWPSNAWVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTE |
Antigen
Chain: D
Mutation: NULL
| >6IEK_D|Chain A, D|NSmGnGc|Rift valley fever virus (11588) EDPHLRNRPGKGHNYIDGMTQEDATCKPVTYAGACSSFDVLLEKGKFPLFQSYAHHRTLLEAVHDTIIAKADPPSCDLQSAHGNPCMKEKLVMKTHCPNDYQSAHYLNNDGKMASVKCPPKYELTEDCNFCRQMTGASLKKGSYPLQDLFCQSSEDDGSKLKTKMKGVCEVGVQALKKCDGQLSTAHEVVPFAVFKNSKKVYLDKLDLKTEENLLPDSFVCFEHKGQYKGTIDSGQTKRELKSFDISQCPKIGGHGSKKCTGDAAFCSAYECTAQYANAYCSHANGSGIVQIQVSGVWKKPLCVGYERVVVKRELS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: TYR27 GLU31 ASP52 GLU54 ASP55 ASP99 ILE101 ARG102 F: ILE28 SER29 SER31 SER32 TYR33 ASN34 TYR53 SER54 ASP55 SER56 ASP57 ALA74 TRP99 PRO100 SER101 D: LYS11 HIS13 TYR15 THR20 GLN21 GLU22 ASP23 ALA24 LYS27 PRO28 GLN102 PRO120 LYS121 MET134 ALA137 SER138 LEU139 LYS140 LYS141 LYS242 GLN248 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
