Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2301 |
| PDBID: | 6IAP |
| Chains: | ED_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1016/j.cell.2019.04.041 |
| Antibody | |
| Antibody: | NKp46-4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Natural cytotoxicity triggering receptor 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6IAP_E|Chain C[auth E]|Fab NKp46-4 heavy chain|synthetic construct (32630) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSFTMHWVRQAPGQGLEWIGYINPSSGYTEYNQKFKDRVTITADKSTSTAYMELSSLRSEDTAVYYCVRGSSRGFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEP |
Light Chain: D
Mutation: NULL
| >6IAP_D|Chain B[auth D]|Fab NKp46-4 light chain|synthetic construct (32630) DIQMTQSPSSLSASVGDRVTITCRASENIYSNLAWFQQKPGKAPKLLVYAATNLADGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQHFWGTPRTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: A
Mutation: NULL
| >6IAP_A|Chain A|Natural cytotoxicity triggering receptor 1|Homo sapiens (9606) TLPKPFIWAEPHFMVPKEKQVTICCQGNYGAVEYQLHFEGSLFAVDRPKPPERINKVKFYIPDMNSRMAGQYSCIYRVGELWSEPSNLLDLVVTEMYDTPTLSVHPGPEVISGEKVTFYCRLDTATSMFLLLKEGRSSHVQRGYGKVQAEFPLGPVTTAHRGTYRCFGSYNNHAWSFPSEPV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TYR30 ASN32 PHE91 TRP92 GLY93 THR94 ARG96 E: SER31 PHE32 THR33 HIS35 TYR50 ASN52 SER54 SER55 TYR57 GLU59 GLY99 SER100 SER101 GLY103 PHE104 A: VAL32 GLU33 GLN35 HIS37 GLY40 LEU42 ARG47 PRO48 LYS49 PRO50 PRO51 GLU52 SER73 ILE75 ARG77 VAL78 GLY79 GLU80 LEU81 TRP82 SER83 GLU84 PRO85 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
