Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2298 |
| PDBID: | 6I8S |
| Chains: | HL_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/s41598-019-38842-x |
| Antibody | |
| Antibody: | PAI-1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Plasminogen activator inhibitor 1 |
| Antigen mutation: | No |
| Durg Target: | P05121 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6I8S_H|Chain E, F, G, H|HEAVY CHAIN OF FAB FRAGMENT FROM AN PAI-1 ANTIBODY|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIIPTFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCARERRQWLEGHFDYWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPQPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: L
Mutation: NULL
| >6I8S_L|Chain I, J, K, L|LIGHT CHAIN OF FAB FRAGMENT FROM AN PAI-1 ANTIBODY|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASEGIYHNLAWYQQKPGKAPKLLIYKASSLASGVPSRFSGSRSGTEFTLTISSLQPDDFATYYCQQYSNYPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >6I8S_D|Chain A, B, C, D|Plasminogen activator inhibitor 1|Homo sapiens (9606) VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISHLLGTGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVALALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIIDRPFLFVVRHNPTGTVLFMGQVMEP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY50 ILE51 ILE52 PHE55 THR57 ASN59 ARG101 TRP103 LEU104 GLU105 GLY106 L: GLY28 TYR30 HIS31 ASN32 LYS50 ARG66 GLY68 TYR91 SER92 ASN93 TYR94 LEU96 D: THR205 LYS207 TYR210 THR211 GLU212 TYR220 TYR241 THR267 ARG268 LEU269 PRO270 ARG271 LEU272 ALA345 ARG346 MET347 ALA348 PRO349 GLU350 GLU351 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
