Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2294 |
| PDBID: | 6I8H |
| Chains: | B_A |
| Organism: | Arabidopsis thaliana, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.68 |
| Reference: | 10.1016/j.jsb.2019.09.007 |
| Antibody | |
| Antibody: | ENB15 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein EDS1L |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6I8H_B|Chain B|EDS1-specific nanobody|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAGSGRTFSTYDMAWFRQAPGKEREFVSSISSSGGNVVYRDSVKGRFTIARDNAANAVYLQMNSLKPEDTAVYYCAAKWLAADYNYWGQGTQVTVSSAAAYPYDVPDYGSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >6I8H_A|Chain A|Protein EDS1L|Arabidopsis thaliana (3702) MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAVIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVTRFDIVPRITLARKASVEETLPHVLAQLDPRNSSVQESEQRITEFYTSVMRDTSTVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFNHLDGENSIESSLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVIQQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLREWITAGEVDEKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEITDTLEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: SER26 GLY27 ARG28 PHE30 THR36 TYR37 ASP38 ASN64 VAL65 VAL66 TYR67 ARG68 ASP69 LYS72 LYS107 TRP108 LEU109 ALA110 ALA113 ASP114 ASN116 TYR117 TRP118 A: ASP56 ILE64 LYS65 LEU66 ASN67 GLN70 PHE71 VAL82 GLU87 LEU90 LYS91 LEU93 GLU94 ALA95 ILE97 ASP98 PRO99 ARG100 TRP135 GLN529 LEU530 GLU531 GLN534 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
