Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2293 |
| PDBID: | 6I8G |
| Chains: | B_A |
| Organism: | Arabidopsis thaliana, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.34 |
| Reference: | 10.1016/j.jsb.2019.09.007 |
| Antibody | |
| Antibody: | ENB73 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Protein EDS1L |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6I8G_B|Chain B|EDS1-specific nanobody|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCATSTHTAGQYTMAWFRQAPGKEREFVAVLRWSDYSTDYANSVKNRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAGWPVKVISSADEYINWGQGTQVTVSSAAAYPYDVPDYGSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >6I8G_A|Chain A|Protein EDS1L|Arabidopsis thaliana (3702) MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSSFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAVIDPRTSFQASVEMAVRSRKQIVFTGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFVNFVTRFDIVPRITLARKASVEETLPHVLAQLDPRNSSVQESEQRITEFYTSVMRDTSTVANQAVCELTGSAEAILETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTCQASDEQEWSLIPFRSIRDHHSYEELVQSMGMKLFNHLDGENSIESSLNDLGVSTRGRQYVQAALEEEKKRVENQKKIIQVIQQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFKANVKRAELAGVFDEVLGLLKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNEDTGPYMKRGRPTRYIYAQRGYEHHILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNSGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLREWITAGEVDEKEIFLEGSTFRKWWITLPKNHKSHSPLRDYMMDEITDTLEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN1 THR27 HIS28 ALA30 GLY35 GLN36 TYR37 ARG57 TRP58 SER59 TYR63 TRP108 PRO109 ILE116 ASN117 A: ARG16 TRP18 SER19 LYS22 GLN23 TYR25 LEU26 GLU33 HIS122 GLN210 ASP212 PRO213 ARG214 ASN215 SER216 SER217 HIS318 GLU321 GLU322 GLN325 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
