Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2291 |
| PDBID: | 6I3Z |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1172/jci.insight.135459 |
| Antibody | |
| Antibody: | 2H2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Alpha-1-antitrypsin |
| Antigen mutation: | No |
| Durg Target: | P01009 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6I3Z_H|Chain C[auth H]|Fab 2H2 heavy chain|Mus musculus (10090) EVQLEESGGGLVKPGGSLKLSCAASGFAFSIYDMSWVRQTPEKRLEWVAYISSGGGTTYYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAIYYCARHAGVHYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: L
Mutation: NULL
| >6I3Z_L|Chain D[auth L]|Fab 2H2 light chain|Mus musculus (10090) DIVMTQSPSSMYASLGERVTITCKASQDINSYLSWFQQKPGKSPKNLIYRANRLVDGVPSRFSGSGSGQDYSLTISSLEYEDMGIYYCLQYDEFPWTFGGGTKLESKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >6I3Z_A|Chain A|Alpha-1-antitrypsin|Homo sapiens (9606) MRGSDPQGDAAQKTDTSHHDQDHPTFNKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAMLSLGTKADTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNGLFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKGTQGKIVDLVKELDRDTVFALVNYIFFKGKWERPFEVKDTEEEDFHVDQVTTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLENELTHDIITKFLENEDRRSASLHLPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEEAPLKLSKAVHKAVLTIDEKGTEAAGAMFL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER30 ILE31 TYR32 ASP33 TYR50 SER52 GLY53 GLY55 ARG94 ALA96 GLY97 VAL98 L: ASN30 TYR32 TYR49 ARG50 ARG53 ASP56 TYR91 ASP92 PHE94 TRP96 A: VAL200 LYS201 ASP202 THR203 GLU204 GLU205 GLU206 LYS217 VAL218 PRO219 LYS222 LYS290 LEU291 SER292 THR339 ASP341 GLU342 LYS343 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
