Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2287 |
| PDBID: | 6I04 |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1073/pnas.1819085116 |
| Antibody | |
| Antibody: | MM-131 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatocyte growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | P08581 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6I04_H|Chain B[auth C], E[auth H]|Fab heavy chain|Homo sapiens (9606) QLQLQESGPGLVKPSETLSLTCTVSGGSISSSVYYWSWIRQPPGKGLEWIGVIYPSGNTYYSPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARTIYDLFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCAAAHHHHHH |
Light Chain: L
Mutation: NULL
| >6I04_L|Chain C[auth D], F[auth L]|Fab light chain|Homo sapiens (9606) DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCLQANSFPPTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >6I04_A|Chain A, D[auth B]|Hepatocyte growth factor receptor|Homo sapiens (9606) ECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAGGGGGSHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: VAL33 TYR35 TYR54 SER56 ASN58 TYR60 LYS66 ILE101 TYR102 ASP103 LEU104 ASP106 L: SER30 SER31 TRP32 LEU46 TYR49 ALA50 ALA51 SER52 SER53 LEU54 GLN55 SER56 SER65 GLY66 SER67 GLY68 ALA91 ASN92 SER93 PHE94 A: HIS92 PRO93 ASP94 PHE96 PRO97 CYS98 GLN99 ASP100 SER103 LYS104 ALA105 ASN106 LEU107 SER108 ARG138 HIS159 CYS160 PHE162 SER163 PRO164 GLN165 ILE166 GLU167 PRO169 CYS172 LYS220 THR222 ASP224 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
