Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2286 |
| PDBID: | 6HXW |
| Chains: | CD_B |
| Organism: | Homo sapiens, synthetic construct |
| Method: | XRD |
| Resolution (Å): | 2.78 |
| Reference: | 10.1016/j.celrep.2019.04.091 |
| Antibody | |
| Antibody: | IPH53 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 5'-nucleotidase (CD73) |
| Antigen mutation: | No |
| Durg Target: | P21589 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6HXW_C|Chain C, E[auth H]|IPH53 heavy chain|synthetic construct (32630) QIQLVQSGAEVKKPGASVKVSCKASGYTFASYNMYWVRQAPGQRLEWIGYIDPYNGGSSYNQKFKGRVTLTRDKSASTAYMELSSLRSEDTAVYYCARGYNNYKAWFAYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKHHHHHH |
Light Chain: D
Mutation: NULL
| >6HXW_D|Chain D, F[auth L]|IPH53 light chain|synthetic construct (32630) IQMTQSPSSLSASVGDRVTITCKASQSVTNDVAWYQQKPGKAPKLLIYYASNRYTGVPSRFSGSGYGTDFTFTISSLQPEDIATYYCQQDYSSLTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNR |
Antigen
Chain: B
Mutation: NULL
| >6HXW_B|Chain A, B|5'-nucleotidase|Homo sapiens (9606) WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: THR28 SER31 TYR32 ASN33 ASP52 TYR54 LYS74 ARG98 TYR100 ASN101 ASN102 TYR103 TYR109 D: THR30 ASN31 ASP32 TYR49 TYR50 ASN53 ARG54 THR56 TYR67 TYR92 B: HIS103 ASN106 GLU129 LEU132 LYS133 GLU134 ALA135 LYS136 PHE137 PRO138 SER152 GLN153 SER155 GLY156 LEU157 LEU159 TYR161 LYS162 VAL163 LEU164 PRO165 VAL170 LEU210 VAL212 ASP323 LYS326 TRP327 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
