Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2282 |
| PDBID: | 6HIG |
| Chains: | HL_B |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1084/jem.20182359 |
| Antibody | |
| Antibody: | NB01a Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Programmed cell death protein 1 (PD-1) |
| Antigen mutation: | No |
| Durg Target: | Q15116 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6HIG_H|Chain A[auth H]|Heavy Chain|Mus musculus (10090) EVQLVQSGAEVKKPGESLKISCKGSGYSFTNFYIHWVRQAPGQRLEWMGSIYPNYGDTAYNQKFKDRFVFSLDTSVSTAYLQISSLKAEDTAVYYCARGYSYAMDYWGQGTTVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPPCPSCPAPEFLG |
Light Chain: L
Mutation: NULL
| >6HIG_L|Chain B[auth L]|Light Chain|Mus musculus (10090) DIQMTQSPSSVSASVGDRVTITCSASQGISGDLNWYQQKPGKAPKLLIYHTSSLHSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQYYSKDLLTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6HIG_B|Chain C[auth B]|Programmed cell death protein 1|Homo sapiens (9606) NPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDSRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN31 PHE32 TYR33 TYR52 TYR55 ASP57 ARG98 TYR100 SER101 TYR102 ALA103 ASP105 L: ASP32 LEU46 TYR49 HIS50 SER53 LEU54 HIS55 SER56 GLY57 B: ASN33 PRO34 THR36 PHE37 SER38 PRO39 ALA40 LEU41 LEU42 VAL43 THR53 GLU136 SER137 LEU138 ARG139 GLU141 ARG143 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
