Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2281 |
| PDBID: | 6HHD |
| Chains: | B_A |
| Organism: | Mus musculus, Camelus dromedarius |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1371/journal.ppat.1008139 |
| Antibody | |
| Antibody: | 484 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mouse Prion Protein |
| Antigen mutation: | No |
| Durg Target: | P04156 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6HHD_B|Chain B|Nanobody 484|Camelus dromedarius (9838) QVQLQESGGGLVQPGGSLRLSCAASGRTFSSYNMGWFRQAPGKGREFVASITSSGDKSDYTDSVKGRFTISRDNAKNTMYLQMNNLKPEDTATYYCARGLGIYIIRARGGYDHWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >6HHD_A|Chain A|Major prion protein|Mus musculus (10090) GAVVGGLGGYMLGSAMSRPMIHFGNDWEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCVTQYQKESQAYY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: LYS43 ARG45 GLU46 PHE47 ASP59 THR61 ASP62 LEU100 GLY101 ILE102 TYR103 ILE104 ILE105 ARG106 ALA107 ARG108 GLY109 GLY110 A: VAL122 GLY123 GLY124 LEU125 TYR128 ARG164 ASP167 GLN168 TYR169 SER170 ASN171 ASN173 ASN174 HIS177 ASP178 ASN181 ILE182 LYS185 VAL189 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
