Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2277 |
| PDBID: | 6HF1 |
| Chains: | EF_D |
| Organism: | Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.94 |
| Reference: | 10.1002/pro.3537 |
| Antibody | |
| Antibody: | 316 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Quiescin sulfhydryl oxidase 1 (QSOX1) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >6HF1_E|Chain C[auth B], F[auth E]|Fab 316 light chain|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTISCSASSSVSYMYWYHQKPGSSPKPWIYRTSNLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCQQYHSYPLTFGAGTKLELKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Light Chain: F
Mutation: NULL
| >6HF1_F|Chain E[auth F]|Fab 316 heavy chain|Mus musculus (10090) QVQLQQSGPELVKPGASVKISCKASGYSFTSYYIHWVKQRPGQGLEWIGWIYPGSYNTEYSEKFKGKATLTADTSSSTAYMQLSSLTSEDSAVYYCARSEDWFAYWGQGTLVTVSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPK |
Antigen
Chain: D
Mutation: H39A/P86T
| >6HF1_D|Chain A, D|Sulfhydryl oxidase 1|Mus musculus (10090) VLYSSSDPLTLLDADSVRPTVLGSSSAWAVEFFASWCGACIAFAPTWKELANDVKDWRPALNLAVLDCAEETNSAVCREFNIAGFTTVRFFQAFTKNGSGATLPGAGANVQTLRMRLIDALESHRDTWPPACPPLEPAKLNDIDGFFTRNKADYLALVFEREDSYLGREVTLDLSQYHAVAVRRVLNTESDLVNKFGVTDFPSCYLLLRNGSVSRVPVLVESRSFYTSYLRGLPGLTR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: TYR33 TRP50 TYR52 SER55 ASN57 THR58 GLU59 GLU100 ASP101 E: SER30 TYR31 TYR33 ARG49 TYR90 HIS91 SER92 TYR93 D: VAL37 LEU38 ASP43 SER71 TRP72 CYS73 GLY74 ALA75 ILE77 ALA78 PHE79 PRO81 THR82 LYS84 GLU85 ALA142 GLY143 ALA144 ASN145 VAL146 THR148 ARG152 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
