Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2262 |
| PDBID: | 6H5N |
| Chains: | FE_D |
| Organism: | Plasmodium falciparum 3D7, Rattus norvegicus |
| Method: | XRD |
| Resolution (Å): | 3.23 |
| Reference: | 10.1038/s41467-018-06340-9 |
| Antibody | |
| Antibody: | 85RF45.1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Gametocyte surface protein P45/48 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: F
Mutation: NULL
| >6H5N_F|Chain C, F|Antibody 85RF45.1 heavy chain|Rattus norvegicus (10116) EVQLVESGGGLLQPGRSLKLSCVASGFTFNNYWMSWIRQAPGKGLEWIASISNIGGTIYYPDSVKGRFTISRDSAQNTLYLQMNSLRSEDTATYYCTRDLRMSDYFDYWGQGVMVTVSSAETTAPSVYPLAPGTALKSNSMVTLGCLVKGYFPEPVTVTWNSGALSSGVHTFPAVLQSGLYTLTSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRN |
Light Chain: E
Mutation: NULL
| >6H5N_E|Chain B, E|Antibody 85RF45.1 light chain|Rattus norvegicus (10116) QFVLSQPNSVSTNLGSTVKLSCKRSTGNIGSNYVSWYQHHEGRSPTTMIYRDDQRPDGVPDRFSGSIDRSSNSALLTIDNVQTEDEAAYFCHSYSTGMYIFGGGTKLTVLGQPKSTPTLTMFPPSPEELQENKATLVCLISNFSPSGVTVAWKANGTPITQGVDTSNPTKEDNKYMASSFLHLTSDQWRSHNSFTCQVTHEGNTVEKSLSPA |
Antigen
Chain: D
Mutation: NULL
| >6H5N_D|Chain A, D|Gametocyte surface protein P45/48|Plasmodium falciparum (36329) KVIHGCNFSSNVSSKHTFTDSLDISLVDDSAHISCNVHLSEPKYNHLVGLNCPGDIIPDCFFQVYQPESEELEPSNIVYLDSQINIGDIEYYEDAEGDDKIKLFGIVGSIPKTTSFTCICKKDKKSAYMTVTIDSA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: GLY30 SER31 ASN32 TYR33 ASP52 ARG69 TYR94 SER95 THR96 F: ASN30 ASN31 TRP33 SER52 ASN53 ILE54 GLY56 THR57 TYR59 ARG101 MET102 ASP104 D: ASP321 SER322 HIS324 PRO345 GLY346 ASP347 ILE348 ILE349 PRO350 ASP351 PHE354 GLN355 ILE369 TYR371 GLU385 ASP390 LYS392 LYS394 ILE411 LYS413 LYS414 ASP415 LYS416 SER418 TYR420 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
