Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2259 |
| PDBID: | 6H3U |
| Chains: | HL_B |
| Organism: | Mus musculus, Bovine Schmallenberg virus BH80/Germany/2011 |
| Method: | XRD |
| Resolution (Å): | 3.17 |
| Reference: | 10.1038/s41467-019-08832-8 |
| Antibody | |
| Antibody: | 4B6 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Schmallenberg Virus Envelopment polyprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6H3U_H|Chain A[auth H], F[auth I]|scFv 4B6 VH|Mus musculus (10090) QMQLQESGPGLVKPSQSLSLTCTVTGYSITSDSAWNWIRQFPGNKLEWMGYISYSGSTSYNPSLKSRISITRDTSKNQFFLQLNSVTTEDTATYYCTRWGDGYYLYWYFDVWGAGTTVTVSSGGGGSGGGGSGGGGSGGGGG |
Light Chain: L
Mutation: NULL
| >6H3U_L|Chain B[auth L], E[auth M]|scFv 4B6 VL|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCSASQGISYYLNWYQQKPDGTVKLLIYYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQQYSKLPWTFGGGTKLEIKGGWSHPQFEK |
Antigen
Chain: B
Mutation: NULL
| >6H3U_B|Chain C[auth A], D[auth B]|Envelopment polyprotein|Bovine Schmallenberg virus (1318464) QETSINCKNIQSTQLTIEHLSKCMAFYQNKTSSPVVINEIISDASVDEQELIKSLNLNCNVIDRFISESSVIETQVYYEYIKSQLCPLQVHDIFTINSASNIQWKALARSFTLGVCNTNPHKHICRCLESMQMCTSTKTDHAREMSIYYDGHPDRFEHDMKIILNIMRYIVPGLGRVLLDQIKQTKDYQALRHIQGKLSPKSQSNLQLKGFLEFVDFILGANVTIEKTPQTLTTLSLIGGWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER31 ASP32 TYR53 SER54 ASP97 GLY98 TYR99 L: GLY28 SER30 TYR31 TYR32 TYR49 TYR50 SER52 SER53 GLY68 SER92 LYS93 B: SER496 SER497 PRO498 VAL500 ILE501 ASN502 ILE505 GLN513 LYS517 SER518 ASN520 LEU521 ASN522 CYS523 ASN524 VAL525 LEU549 CYS550 PRO551 LEU552 GLN553 ASP556 THR559 ALA563 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
