Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2258 |
| PDBID: | 6H3T |
| Chains: | IM_B |
| Organism: | Mus musculus, Bovine Schmallenberg virus BH80/Germany/2011 |
| Method: | XRD |
| Resolution (Å): | 2.84 |
| Reference: | 10.1038/s41467-019-08832-8 |
| Antibody | |
| Antibody: | 1C11 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Schmallenberg Virus Envelopment polyprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >6H3T_I|Chain A[auth H], D[auth I]|scFv 1C11 VH|Mus musculus (10090) EVQLQQSGPELVKPGASVKVSCKASGYSFTDYNMCWVKQSHGKSLEWIGYIDPYNGLTSYNPKFKGKATLTVDKSSSTAFMHLNSLTSEDSAVYYCARNYYGRSYAMDYWGQGTSVTVSSGGGGSGGGGSGGGGSGGGGG |
Light Chain: M
Mutation: NULL
| >6H3T_M|Chain B[auth L], E[auth M]|scFv 1C11 VL|Mus musculus (10090) DIVMTQAASSLAVSVGEKVTMSCKSSQSLLYSSNQKNYLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYYSFPPTFGGGTKLEIKGGWSHPQFEK |
Antigen
Chain: B
Mutation: NULL
| >6H3T_B|Chain C[auth A], F[auth B]|Envelopment polyprotein|Bovine Schmallenberg virus (1318464) QETSINCKNIQSTQLTIEHLSKCMAFYQNKTSSPVVINEIISDASVDEQELIKSLNLNCNVIDRFISESSVIETQVYYEYIKSQLCPLQVHDIFTINSASNIQWKALARSFTLGVCNTNPHKHICRCLESMQMCTSTKTDHAREMSIYYDGHPDRFEHDMKIILNIMRYIVPGLGRVLLDQIKQTKDYQALRHIQGKLSPKSQSNLQLKGFLEFVDFILGANVTIEKTPQTLTTLSLIGGWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: THR30 ASP31 ASN33 TYR50 TYR53 ASN54 LEU56 THR57 SER58 TYR97 GLY98 M: SER27 ASN28 LYS30 TYR32 TRP50 TYR91 TYR92 SER93 PHE94 B: ARG573 SER574 PHE575 THR576 CYS580 ASN581 THR582 ASN583 PRO584 HIS585 CYS589 ARG590 LEU592 GLU593 SER610 ILE611 TYR612 ASP614 GLY615 HIS616 ASP618 ARG619 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
