Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2254 |
| PDBID: | 6H16 |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1038/s41467-018-08172-z |
| Antibody | |
| Antibody: | L-P2-D07 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Low-density lipoprotein receptor-related protein 6 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6H16_B|Chain B|VHH L-P2-D07|Lama glama (9844) EVQLQESGGGLVQAGGSLRLSCAASGRTFSIYTIGWFRQAPGKEREFVAEITWSGGSTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAAITYTRGIYKYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >6H16_A|Chain A|Low-density lipoprotein receptor-related protein 6|Homo sapiens (9606) PEAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVVEFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGKPKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSNMLGLNREVIADDLPHPFGLTQYQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQSGWNECASSNGHCSHLCLAVPVGGFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLYWIDSRQNMIRKAQEDGSQGFTVVVSSVPSQNLEIQPYDLSIDIYSRYIYWTCEATNVINVTRLDGRSVGVVLKGEQDRPRAVVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSRLGKLFWADSDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSDIHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGAAA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLU2 ARG28 PHE30 SER31 ILE32 TYR33 THR53 TRP54 SER55 GLY57 SER58 ILE100 THR101 TYR102 THR103 ARG104 GLY105 ILE106 TYR107 LYS108 A: ARG639 GLU663 ILE681 SER682 TYR706 GLU708 THR724 GLY725 SER749 ARG751 TRP767 GLY768 ARG792 LEU810 ASP811 ASN813 PRO833 HIS834 PHE836 TRP850 SER851 ASP874 TYR875 MET877 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
