Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2252 |
| PDBID: | 6H15 |
| Chains: | C_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1038/s41467-018-08172-z |
| Antibody | |
| Antibody: | L-P2-B10 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Low-density lipoprotein receptor-related protein 6 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >6H15_C|Chain B[auth C], C[auth D]|VHH L-P2-B10|Lama glama (9844) VQLQESGGCLVQAGGSLRLSCAASGSTFSTYTIGWFRQAPGKEREFVAAIHWDGGQTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAARGRRYFDFTYSDVYDYWGQGTQVTVS |
Antigen
Chain: A
Mutation: NULL
| >6H15_A|Chain A, D[auth B]|Low-density lipoprotein receptor-related protein 6|Homo sapiens (9606) PEAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVVEFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGKPKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSNMLGLNREVIADDLPHPFGLTQYQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQSGWNECASSNGHCSHLCLAVPVGGFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLYWIDSRQNMIRKAQEDGSQGFTVVVSSVPSQNLEIQPYDLSIDIYSRYIYWTCEATNVINVTRLDGRSVGVVLKGEQDRPRAVVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSRLGKLFWADSDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSDIHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: PHE48 ASP55 GLY56 GLN58 TYR60 TYR61 ASP63 LYS66 ARG100 GLY101 ARG102 ARG103 TYR104 PHE105 ASP106 PHE107 THR108 TYR109 ASP111 VAL112 ASP114 A: ARG638 ARG639 ALA640 LYS662 GLU663 ILE681 SER682 TYR706 GLU708 ARG751 TRP767 ARG792 LEU810 ASP811 ASN813 HIS834 PHE836 TRP850 ARG853 ASP874 TYR875 MET877 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
