Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2249 |
| PDBID: | 6GLX |
| Chains: | CD_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1126/science.aaw4295 |
| Antibody | |
| Antibody: | 4E8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Galectin-10 |
| Antigen mutation: | No |
| Durg Target: | Q05315 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6GLX_C|Chain C, E[auth H]|Fab 4E8 - Heavy chain|Lama glama (9844) QVQRQESGPGLVKPSQTLSLTCTVSGGSITTSYYAWSWIRQPPGKGLEWMGVIASDGSTYYSPSLKSRTSISRDTSKNQFSLQLSSVTPEDTAVYYCALYIRGSSWSGWSAYDYWGQGTQVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: D
Mutation: NULL
| >6GLX_D|Chain D, F[auth L]|Fab 4E8 - Light chain|Lama glama (9844) QPVLNQPSAVSVSLGQTARITCQGGNFGYYYGSWYQQKPGQAPVLVIYKDSERPSGIPERFSGSSSGGTATLTISRAQAEDEADYYCQSADSSDNPVFGGGTHLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: B
Mutation: NULL
| >6GLX_B|Chain A, B|Galectin-10|Homo sapiens (9606) MASTTHHHHHHDTDIPTTGGGSRPDDDDKENLYFQGHMSLLPVPYTEAASLSTGSTVTIKGRPLACFLNEPYLQVDFHTEMKEESDIVFHFQVCFGRRVVMNSREYGAWKQQVESKNMPFQDGQEFELSISVLPDKYQVMVNGQSSYTFDHRIKPEAVKMVQVWRDISLTKFNVSYLKR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLY57 SER58 THR59 TYR60 SER104 SER105 SER107 TRP109 D: ASN26 GLY28 TYR29 TYR30 TYR31 LYS49 SER65 ASP91 SER92 SER93 ASP94 B: THR42 ASP49 GLU68 TYR69 LYS73 GLN74 PRO97 ASP98 ASP113 HIS114 ARG115 ILE116 LYS117 PRO118 ALA120 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
