Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2232 |
| PDBID: | 6GK4 |
| Chains: | F_D |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.91 |
| Reference: | 10.1038/s41467-019-10714-y |
| Antibody | |
| Antibody: | D12 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cystic fibrosis transmembrane conductance regulator |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >6GK4_F|Chain C, F|Nanobody T8|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAASGSTSSINAMGWYRQAPGKQREPVAISSSGGDTRYAEPVKGRFTISRDNAQNKVYLQMNSLKPEDTAVYYCWLNWGRTSVNSWGQGTQVTVSSAAAHHHHHHGAAEQKLISEEDLNGAA |
Antigen
Chain: D
Mutation: NULL
| >6GK4_D|Chain A, D|Cystic fibrosis transmembrane conductance regulator|Homo sapiens (9606) SLTTTEVVMENVTAFWEEGGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: ASN32 ALA33 TYR37 GLN44 PRO47 VAL48 ILE50 SER52 SER53 GLY54 GLY55 ASP56 ARG58 TYR59 ALA60 ASN73 TRP96 ASN98 GLY100 ARG101 THR102 SER103 ASN105 TRP107 D: MET469 MET472 GLU474 ILE488 SER489 PHE490 SER492 PHE494 SER495 TRP496 ILE497 MET498 PRO499 GLU504 ASN505 ILE506 PHE508 GLY509 VAL510 VAL540 LEU541 GLY542 GLU543 ARG553 ARG560 LYS564 TYR577 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
