Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2228 |
| PDBID: | 6GJU |
| Chains: | C_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1038/s41467-019-10714-y |
| Antibody | |
| Antibody: | T2a Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cystic fibrosis transmembrane conductance regulator |
| Antigen mutation: | No |
| Durg Target: | P13569; P13569; |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >6GJU_C|Chain C|Nanobody T2a|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGSIFRIDAMGWYRQAPGKQRELVAHSTSGGSTDYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNADVRTRWYASNNYWGQGTQVTVSSAAAHHHHHHGAAEQKLISEEDLNGAA |
Antigen
Chain: A
Mutation: NULL
| >6GJU_A|Chain A|Cystic fibrosis transmembrane conductance regulator|Homo sapiens (9606) SLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCPQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFPEKDNTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition C: ASP32 ALA33 TYR37 ARG45 GLU46 LEU47 HIS50 THR52 SER56 ASP58 ASP61 LYS64 ASN96 ASP98 ARG100 ARG102 TRP103 TYR104 ALA105 SER106 ASN107 ASN108 TRP110 A: ALA457 GLY458 SER459 SER549 GLY550 GLY551 GLY576 TYR577 LEU578 ASP579 VAL580 LEU581 GLU583 SER605 LYS606 MET607 LEU610 LYS611 ILE618 LEU619 HIS620 LEU633 GLN634 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
