Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2227 |
| PDBID: | 6GJU |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1038/s41467-019-10714-y |
| Antibody | |
| Antibody: | T4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cystic fibrosis transmembrane conductance regulator |
| Antigen mutation: | No |
| Durg Target: | P13569; P13569; |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6GJU_B|Chain B|Nanobody T4|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAASGSTFAIIAMGWYRQAPGKQRELVAVISTGDTRYADSVKGRFTISRDNAKNTVYLQMDSLRPEDTAVYYCNAAVQVRDYRNYWGQGTQVTVSSAAAHHHHHHGAAEQKLISEEDLNGAA |
Antigen
Chain: A
Mutation: NULL
| >6GJU_A|Chain A|Cystic fibrosis transmembrane conductance regulator|Homo sapiens (9606) SLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCPQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFPEKDNTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ILE32 ALA33 GLY35 TYR37 LEU47 VAL48 ALA49 VAL50 SER52 THR53 GLY54 ASP55 THR56 ARG57 TYR58 ALA59 ASP60 LYS63 ARG70 ASN95 ALA97 GLN99 TYR103 ARG104 ASN105 TRP107 A: MET469 MET472 GLY473 GLU476 PRO477 ILE488 PHE490 PRO492 PHE494 SER495 TRP496 ILE497 MET498 PRO499 GLU504 ILE506 PHE508 GLY509 VAL510 SER511 GLY542 GLU543 THR547 ARG553 ARG560 ALA561 LYS564 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
