Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2222 |
| PDBID: | 6GJQ |
| Chains: | D_C |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.49 |
| Reference: | 10.1038/s41467-019-10714-y |
| Antibody | |
| Antibody: | T27 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cystic fibrosis transmembrane conductance regulator |
| Antigen mutation: | No |
| Durg Target: | P13569 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >6GJQ_D|Chain B, D, F, H|Nanobody T27|Lama glama (9844) QVQLQESGGGLEQPGGSLRLSCATSGVIFGINAMGWYRQAPGKQRELVATFTSGGSTNYADFVEGRFTISRDNAKNTVYLQMNGLRPEDTAVYYCHATVVVSRYGLTYDYWGQGTQVTVSSAAAHHHHHHGAAEQKLISEEDLNGAA |
Antigen
Chain: C
Mutation: NULL
| >6GJQ_C|Chain A, C, E, G|Cystic fibrosis transmembrane conductance regulator|Homo sapiens (9606) SLTTTEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCPQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFPEKDNTVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: TYR37 GLN44 ARG45 GLU46 LEU47 THR50 THR52 SER53 GLY54 SER56 ASN58 HIS96 THR98 VAL101 ARG103 TYR104 GLY105 LEU106 THR107 TYR108 ASP109 TRP111 C: ALA457 GLY458 SER459 GLY551 GLY576 TYR577 LEU578 ASP579 VAL580 LEU581 GLU585 SER605 LYS606 MET607 GLU608 LEU610 LYS611 ILE618 LEU619 HIS620 TYR625 PHE630 LEU633 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
