Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2208 |
| PDBID: | 6FN1 |
| Chains: | CB_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.58 |
| Reference: | 10.1073/pnas.1717044115 |
| Antibody | |
| Antibody: | UIC2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human-mouse chimeric ABCB1 (ABCB1HM) |
| Antigen mutation: | No |
| Durg Target: | P08183 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >6FN1_C|Chain C|UIC2 Antigen Binding Fragment Heavy Chain|Mus musculus (10090) EVQLQESGPELVKTGASVKISCKASGYSFSNYYIHWVKQSHGKSLEWIGFISCYNGATFYNQKFKGKATFTVDNSSSTAYMKFNSLTFEDSAVYYCARLPIQFGNFYPMDYWGQGTTVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPT |
Light Chain: B
Mutation: NULL
| >6FN1_B|Chain B|UIC2 Antigen Binding Fragment Light chain|Mus musculus (10090) QVVMTQSPLSLPVSLGDQASISCRSSQSLLHSNGNTYLHWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHIPPWTFGGGTKLDIKRADAAPTVSIFPPSSEQLTSGGLSVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >6FN1_A|Chain A|Human-mouse chimeric ABCB1 (ABCBHM)|Homo sapiens (9606) PAVSVLTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLIFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTTYAYYYTGIGAGVLIVAYIQVSFWLLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGAGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATCALDTESEAVVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFDGGVIVEQGNHDELMREKGIYFKLVMTQTAGNEIELGNEAAKSKDEIDNLDMSSKDSGSSLIRRRSTRKSIAGPHDQDRKLSTKEALDEDVPPASFWRILKLNSTEWPYFVVGIFVAIINGGLQPAFSVIFSKIIGVFTRIDDPETKRQNSNLFSLLFLILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAMMYFSYAAAFRFGAYLVAHKLMSFEDVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGAGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDTSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATCALDTESEKVVQEALDKAREGRTTIVIAHRLSTIQNADLIVVIQNGKVKEHGTHQQLLAQKGIYFSMVSVQAGAKRS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASN33 ASN35 TYR37 LYS55 ASN58 HIS98 ILE99 PRO100 TRP102 C: TYR27 SER28 ASN31 TYR32 TYR33 PHE50 SER52 TYR54 ASN55 ALA57 THR58 PHE59 GLN62 PRO100 ILE101 GLN102 PHE103 GLY104 ASN105 PHE106 TYR107 A: GLU73 ASP76 ILE77 ALA79 ASN80 ALA81 ASN83 LEU84 LEU87 MET88 ASN90 ILE91 ASN98 PHE102 ASN105 ASP109 VAL320 LEU321 SER322 GLY323 GLU324 TYR325 SER326 ILE327 PHE738 ARG740 ILE741 ASP742 ASP743 PRO744 GLU745 THR746 LYS747 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
