Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2196 |
| PDBID: | 6FAX |
| Chains: | HL_R |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.99 |
| Reference: | 10.1016/j.ccell.2018.02.009 |
| Antibody | |
| Antibody: | Lob 7.4 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tumor necrosis factor receptor superfamily member 5 (CD40) |
| Antigen mutation: | No |
| Durg Target: | P25942 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6FAX_H|Chain B[auth H]|Lob 7.4 heavy chain|Homo sapiens (9606) EVQLQQSGPDLVKPGASVKISCKTSGYTFTEYIMHWVKQSHGKSLEWIGGIIPNNGGTSYNQKFKDKATMTVDKSSSTGYMELRSLTSEDSAVYYCTRREVYGRNYYALDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKCCDKTHTCPPCPAPELL |
Light Chain: L
Mutation: NULL
| >6FAX_L|Chain A[auth L]|Lob 7.4 light chain|Homo sapiens (9606) DIQMTQTTSSLSASLGDRVTITCSASQGINNYLNWYQQKPDGTVKLLIYYTSSLHSGVPSRFSGSGSGTDYSLTISNLEPEDIATYYCQQYSNLPYTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: R
Mutation: NULL
| >6FAX_R|Chain C[auth R]|Tumor necrosis factor receptor superfamily member 5|Homo sapiens (9606) EPPTACREKQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCGESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSETDTICTCEEGWHCTSEACESCVLHRSCSPGFGVKQIATGVSDTICEPCPVGFFSNVSSAFEKCHPWTSCETKDLVVQQAGTNKTDVVCGPQDRLR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN30 ASN31 TYR32 TYR49 TYR50 SER53 TYR91 SER92 ASN93 LEU94 TYR96 H: THR28 GLU31 ILE33 HIS35 TRP47 GLY50 ILE52 ASN55 THR58 SER59 ARG99 VAL101 TYR102 GLY103 ARG104 ASN105 TYR106 TYR107 R: GLU21 PRO22 PRO23 ARG27 LYS29 GLN30 SER35 GLN36 CYS37 SER39 LEU40 GLN42 GLN45 CYS51 THR52 GLU53 PHE54 THR55 GLU56 THR57 CYS59 TRP71 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
