Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2193 |
| PDBID: | 6EYO |
| Chains: | IM_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/s41598-018-29664-4 |
| Antibody | |
| Antibody: | 8D6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Immunoglobulin heavy constant epsilon |
| Antigen mutation: | No |
| Durg Target: | P01854 |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >6EYO_I|Chain C[auth H], D[auth I]|8D6 Fab heavy chain|Homo sapiens (9606) QVQLQQSGAELAKPGASVMLSCKASGYTFNGYWMHWVKQRPGQDLEWIGYINPTTGHTEYNQKFKDKATLTADESSNTAYIELSSLTSDDSAVYYCARQEYRHSWFAYWGQGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKAEPKSCDKTHT |
Light Chain: M
Mutation: NULL
| >6EYO_M|Chain E[auth L], F[auth M]|8D6 Fab light chain|Homo sapiens (9606) DIVLTQSPASLAVSLGQRATISCKASQSVDYDGDTYMNWYHQKPGQPPKLLIYAASNLDSGIPARFSGSGSGTDFTLNIHPVEEEDAATYYCQQTNEDPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >6EYO_B|Chain A, B|Immunoglobulin heavy constant epsilon|Homo sapiens (9606) DIVASRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTIQITWLEDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVQLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: TYR27 PHE29 ASN30 GLY31 TYR32 TRP33 HIS35 TYR50 ASN52 THR54 THR55 HIS57 GLU74 GLN99 TYR101 ARG102 HIS103 SER104 M: TYR31 TYR36 THR95 ASN96 GLU97 ASP98 TRP100 B: PRO249 GLN301 LYS302 LEU305 ARG334 ASP362 LEU363 ALA364 PRO365 SER366 LYS367 GLY368 THR369 ARG387 GLU389 GLU390 LYS391 ARG393 ASN394 GLY395 THR396 LEU397 HIS424 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
