Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 218 |
| PDBID: | 2B2X |
| Chains: | IM_B |
| Organism: | Rattus norvegicus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1110/ps.052030506 |
| Antibody | |
| Antibody: | AQC2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Integrin alpha-1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >2B2X_I|Chain B[auth H], E[auth I]|Antibody AQC2 Fab|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYTMSWVRQAPGKGLEWVAVISGGGHTYYLDSVEGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCTRGFGDGGYFDVWGQGTLVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCHHHHHH |
Light Chain: M
Mutation: NULL
| >2B2X_M|Chain C[auth L], F[auth M]|Antibody AQC2 Fab|Mus musculus (10090) QIQLTQSPSSLSASVGDRVTITCSASSQVNHMFWYQQKPGKAPKPWIYLTSYLASGVPSRFSGSGSGTDYTLTISSLQPEDFATYYCQQWSGNPWTFGQGTKVEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: NULL
| >2B2X_B|Chain A, D[auth B]|Integrin alpha-1|Rattus norvegicus (10116) GSVSPTFQVVNSFAPVQECSTQLDIVIVLDGSNSIYPWESVIAFLNDLLKRMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAANKIVQRGGRQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKALGERIFALEALERPHRD |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: SER30 ARG31 THR33 TRP47 VAL50 SER52 GLY53 GLY54 HIS56 TYR58 TYR59 PHE99 GLY100 ASP101 M: GLN28 VAL29 ASN30 HIS31 LEU49 TYR52 TRP90 SER91 ASN93 PRO94 TRP95 B: SER156 ASN157 SER158 ILE159 TYR160 PRO161 GLU192 GLN218 ARG219 GLY220 GLY221 ARG222 GLN223 THR224 MET225 GLU259 SER260 HIS261 ASN263 TYR264 HIS288 TYR289 SER295 LYS298 GLU321 LEU322 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
