Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2164 |
| PDBID: | 6DZV |
| Chains: | BC_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 4.20 |
| Reference: | 10.1038/s41586-019-1135-1 |
| Antibody | |
| Antibody: | 15B8 Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Sodium-dependent serotonin transporter |
| Antigen mutation: | No |
| Durg Target: | P31645 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6DZV_B|Chain B|15B8 antibody heavy chain|Mus musculus (10090) QVQLQQSGPELVKLGASVRISCKASGYRFSYSWMNWVKQRPGKGLEWIGRIYPGDGDTKYSGKFKGKATLTADKSSSTVYMQLSSLTSEDSAVYFCARSAYGSEGFAMDYWGQGTSVT |
Light Chain: C
Mutation: NULL
| >6DZV_C|Chain C|15B8 antibody light chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASESVDNYGISFLNWFQQKPGQPPKLLIYAASNQGSGVPARFSGSGSGTYFSLNIHPMEEDDTAVYFCQQTKGVSWTFGGGTKVEI |
Antigen
Chain: A
Mutation: NULL
| >6DZV_A|Chain A|Sodium-dependent serotonin transporter|Homo sapiens (9606) RETWGKKVDFLLSVIGYAVDLGNVWRFPYICYQNGGGAFLLPYTIMAIFGGIPLFYMELALGQYHRNGCISIWRKICPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCTNYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKVVWVTATFPYIILSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASYNKFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPASTFFAIIFFLMLITLGLDSTFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVKLLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSPPQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: ARG47 TYR50 TRP52 ARG69 TYR71 ASP74 ASP76 LYS78 LYS93 SER118 TYR120 GLY121 SER122 PHE125 C: ASN51 TYR52 GLY53 ILE54 PHE56 THR115 GLY117 TRP120 A: GLN194 THR198 SER199 CYS200 LYS201 ASN202 SER203 TRP204 ASN205 THR206 GLY207 CYS209 THR210 TYR212 SER214 ASN217 ILE218 THR219 ARG234 HIS235 GLN238 HIS240 ARG241 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
