Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2155 |
| PDBID: | 6DDV |
| Chains: | BA_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.05 |
| Reference: | 10.1080/19420862.2018.1532767 |
| Antibody | |
| Antibody: | Anti-MICA clone 6E1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MHC class I chain-related protein A |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6DDV_B|Chain A[auth B]|Anti-MICA Fab fragment heavy chain clone 6E1|Mus musculus (10090) QGQMQQSGAELVKPGASVKLSCKTSGFTFSDNYISWLKQKPGQSLEWIAWIYAGTGGSSYNQKFRDKAQLTVDTSSRTAYMQLSSLTTEDSAIYYCARHDYYGTSGAWFAYWGRGTLVTVSAASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: A
Mutation: NULL
| >6DDV_A|Chain C[auth A]|Anti-MICA Fab fragment light chain clone 6E1|Mus musculus (10090) DVLMTQTPLSLPVSLGDQASISCRSSQHIVHSNENTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPWTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: C70S
| >6DDV_C|Chain B[auth C]|MHC class I chain-related protein A|Homo sapiens (9606) TVPPMVNVTRSEASEGNITVTCRASSFYPRNIILTWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRISRGEEQRFTCYMEHSGNHSTHPVPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ASP31 TYR33 TRP50 THR54 GLY56 SER57 HIS95 TYR97 TYR98 A: SER27 ASN28 ASN30 TYR32 LYS50 C: THR224 ARG226 SER229 ARG233 TRP253 GLY254 ASP255 VAL256 LEU257 PRO258 ASP259 GLY260 ASN261 GLN265 THR266 TRP267 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
