Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2153 |
| PDBID: | 6DDM |
| Chains: | BA_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.30 |
| Reference: | 10.1080/19420862.2018.1532767 |
| Antibody | |
| Antibody: | Anti-MICA clone 1D5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MHC class I polypeptide-related sequence A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >6DDM_B|Chain C[auth B]|Anti-MICA Fab fragment heavy chain clone 1D5|Mus musculus (10090) EIQLQQSGPELVKPGASVKVSCKASGYAFTSQNIYWVKQSHGKSLEWIGYIEPYNVVPMYNPKFKGKATLTVDKSSSSAYIHLNSLTSEDSAIYYCARSGSSNFDYWGQGTTLTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: A
Mutation: NULL
| >6DDM_A|Chain A|Anti-MICA Fab fragment light chain clone 1D5|Mus musculus (10090) EIILTQSPTTMAASPGEKITITCSASSSISSHYLHWYQQKSGFSPKLLIYRTSNLASGVPARFSGSGSGTSYSLTIGTMEAEDVATYYCQQGSSLPLTFGAGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >6DDM_C|Chain B[auth C]|MHC class I polypeptide-related sequence A|Homo sapiens (9606) TVPPMVNVTRSEASEGNITVTCRASSFYPRNIILTWRQDGVSLSHDTQQWGDVLPDGNGTYQTWVATRICRGEEQRFTCYMEHSGNHSTHPVPS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: HIS31 TYR32 HIS34 ARG50 GLY91 SER92 SER93 LEU94 LEU96 B: ALA28 THR30 SER31 GLN32 ASN33 TYR35 TYR50 TYR53 ASN54 VAL56 SER95 GLY96 SER97 SER98 ASN99 C: ARG240 GLN241 ASP242 GLY243 VAL244 SER245 LEU246 SER247 ASP249 THR250 GLN278 ARG279 PHE280 THR281 TYR283 HIS290 SER291 THR292 HIS293 PRO294 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
