Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 215 |
| PDBID: | 2ARJ |
| Chains: | HL_Q |
| Organism: | Rattus norvegicus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.88 |
| Reference: | 10.1016/j.jmb.2006.02.016 |
| Antibody | |
| Antibody: | YTS-105.18 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T cell surface glycoprotein CD8 alpha chain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2ARJ_H|Chain B, D[auth H]|YTS 105.18 antigen binding region Heavy chain|Rattus norvegicus (10116) QVQLKESGPGLVQPSQTLSLTCTVSGFSLTSNSVHWVRQPPGKGLEWMGGIWGDGDTDYNSALKSRLSISRDTSKNQVFLKMNSLQTDDTAIYFCTPLIGSWYFDFWGPGTMVTASSAQTTAPSVYPLAPGCGDTTSSTVTLGCLVKGYFPEPVTVTWNSGALSSDVHTFPAVLQSGLYTLTSSVTSSTWPSQTVTCNVAHPASSTKVDQKIVPR |
Light Chain: L
Mutation: NULL
| >2ARJ_L|Chain A, C[auth L]|YTS 105.18 antigen binding region Light chain|Rattus norvegicus (10116) DIVMTQSPSSLAVSAGERVTLNCKASQNVRNNIAWYQQKPGQSPKLLIYYASYRYTGVPDRFTGDGFGTDFTLAINSVQADDAAFYYCQRIYNSPYTFGAGTKLELIRADAAPTVSIFPPSMEQLTSGGASVVCFVNNFYPRDISVKWKIDGSEQRDGVLDSVTDQDSKDSTYSMSSTLSLTKVEYERHNLYTCEVVHKTSSSPVVKSFNR |
Antigen
Chain: Q
Mutation: NULL
| >2ARJ_Q|Chain E[auth Q], F[auth R]|T-cell surface glycoprotein CD8 alpha chain|Mus musculus (10090) KPQAPELRIFPKKMDAELGQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSAMRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN31 ASN32 TYR49 TYR50 TYR53 THR56 TYR92 ASN93 H: SER28 THR30 SER31 ASN32 TRP52 GLY53 ASP54 ASP56 LEU95 ILE96 GLY97 SER98 TRP99 ASP101 Q: ALA4 GLU6 ARG8 ILE9 PHE10 PRO11 LYS12 LYS13 MET14 ASP15 GLN20 LYS21 VAL22 ASP23 VAL25 GLU27 LEU29 GLY30 ASP80 ASN82 ASN83 LYS84 SER114 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
