Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2131 |
| PDBID: | 6CWT |
| Chains: | AB_E |
| Organism: | Oryctolagus cuniculus, Hepatitis B virus subtype adyw |
| Method: | XRD |
| Resolution (Å): | 3.15 |
| Reference: | 10.1016/j.str.2018.06.012 |
| Antibody | |
| Antibody: | e21 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis B virus Capsid protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >6CWT_A|Chain A, C|Fab e21 heavy chain|Oryctolagus cuniculus (9986) QSVKESEGRLVTPGTPLTLTCTGSGFSLSTYAMIWVRQAPGQGVEYIGIINTGGSASYASWAKGRFTISRTSTTVDLKITSPTTEDTATYFCARTRGVNDAYEHAFDPWGPGTLVTVSSASTKGPSVFPLAPSAKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTRGHHHHHH |
Light Chain: B
Mutation: NULL
| >6CWT_B|Chain B, D|Fab e21 light chain|Oryctolagus cuniculus (9986) AELVMTQTPSSTSAAVGGTVTINCQASQSIGNALAWYQQKPGQPPKLLISAGSNLASGVPSRFRGSGSGTEYTLTISDVQREDAATYYCLGTYSAIDRAFGAGTNVEIERTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: C48A/C107A
| >6CWT_E|Chain E, F|Capsid protein|Hepatitis B virus subtype adyw (10419) MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTAAALYRDALESPEHASPHHTALRQAILCWGDLMTLATWVGTNLEDPASRDLVVSYVNTNVGLKFRQLLWFHISALTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASN51 THR52 GLY53 ARG96 VAL98 ASN99 ASP100 ALA101 TYR102 GLU103 HIS104 B: SER29 ILE30 GLY31 ASN32 ALA33 SER50 ALA51 GLY52 SER53 ASN54 LEU55 ALA56 SER68 GLY69 THR92 TYR93 SER94 ALA95 ILE96 ARG98 E: LYS7 GLY10 ALA11 THR12 GLU14 LEU15 PHE18 ARG112 GLU113 LEU116 GLU117 VAL120 SER121 VAL124 TRP125 ARG127 THR128 PRO129 TYR132 PRO134 PRO135 ASN136 PRO138 ILE139 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
