Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2126 |
| PDBID: | 6CVK |
| Chains: | B_D |
| Organism: | Oryctolagus cuniculus, Hepatitis B virus |
| Method: | XRD |
| Resolution (Å): | 2.38 |
| Reference: | 10.1016/j.str.2018.06.012 |
| Antibody | |
| Antibody: | e13 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hepatitis B virus Capsid protein |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6CVK_B|Chain A, B|Single chain variable fragment (scFv) e13|Oryctolagus cuniculus (9986) AAELVLTQTPSSVSAAVGGTVTINCQASQSISSRLGWYQQKPGQPPKLLIYGASTLTSGVPSRFKGSGSGTEFTLTISGVQRDDAATYYCLGSDTSTDTAFGGGTEVVVKGGSSRSSSSGGGGSGGGGQEQLVESGGRLVPPGGSLTLTCTVSGIDLSSNAISWVRQAPGKGLEYIGIIYGGSIPYYSRWAKGRFTISKTSTTVALKMSTLTASDTATYFCARGKSDGDGYAAYRLDPWGLGTLVTISSLVPRGSHHHHHH |
Antigen
Chain: D
Mutation: C58A/X102N/C117A
| >6CVK_D|Chain C, D|Capsid protein|Hepatitis B virus (10407) SKLCLGWLWGMDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTAAALYRDALESPEHASPHHTALRQAILCWGDLMTLATWVGTNLEDPASRDLVVSYVNTNVGLKFRQLLWFHISALTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: ARG34 ASP94 THR95 SER96 THR97 ILE155 ASP156 LEU157 SER158 SER159 ILE179 TYR180 GLY181 GLY182 SER183 ILE184 PRO185 TYR186 THR196 ILE197 SER198 LYS199 THR200 SER201 LYS207 LYS225 SER226 ASP227 GLY228 ASP229 GLY230 TYR231 D: PRO30 SER31 ASP32 PHE33 PHE34 PRO35 SER36 VAL37 ARG38 ASP39 LEU40 ASP42 THR43 ASN100 ARG108 TRP112 ILE115 THR119 PHE120 TYR128 PHE132 TRP135 PRO145 ASN146 ALA147 ILE149 LEU150 SER151 THR152 GLU155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
