Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 212 |
| PDBID: | 2AEP |
| Chains: | HL_A |
| Organism: | Influenza A virus (A/Memphis/31/98(H3N2)), Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1016/j.jmb.2005.11.061 |
| Antibody | |
| Antibody: | NA Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | H3N2 neuraminidase |
| Antigen mutation: | No |
| Durg Target: | P06818 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2AEP_H|Chain B[auth H]|FAB heavy chain|Mus musculus (10090) EVKLVESGGGLVQPGGSLSLSCATSGFTFIDYYMSWFRQPPGKALEWLGLIRNKGNGYTMEYSASLKGRFTISRDNSQSIVYLHMNTLTAEDSATYYCARVDYGTNYDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKI |
Light Chain: L
Mutation: NULL
| >2AEP_L|Chain C[auth L]|FAB light chain|Mus musculus (10090) DILMTQSQKFLSTSVGDRVSVTCKASQNVGTNVAWYQKKPGQSPKPLMYSASYRYSGVPDRFTGSGSGTDFTLTISNVQSEDLAEYFCQQFNRYPLTFGSGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >2AEP_A|Chain A|neuraminidase|Influenza A virus (228928) KEICPKLAEYRNWSKPQCKITGFAPFSKDNSIRLSAGGDIWVTREPYVSCDPDKCYQFALGQGTTLNNRHSNDTVHDRTPYRTLLMNELGVPFHLGTKQVCIAWSSSSCHDGKAWLHVCVTGHDENATASFIYDGRLVDSIGSWSKKILRTQESECVCINGTCTVVMTDGSASGRADTKILFIEEGKIVHISPLSGSAQHVEECSCYPRYPGVRCVCRDNWKGSNRPIVDINVKDYSIVSSYVCSGLVGDTPRKNDSSSSSHCLNPNNEEGGHGVKGWAFDDGNDVWMGRTISEKFRSGYETFKVIEGWSKPNSKLQINRQVIVDRGNRSGYSGIFSVEGKSCINRCFYVELIRGRKQETEVWWTSNSIVVFCGTSGTYGTGSWPDGADINLMPI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 ILE30 ASP31 TYR33 GLY52 ASN53 THR56 GLU58 ASP96 TYR97 GLY98 THR99 L: THR31 ASN32 TYR49 SER50 SER52 TYR53 ARG54 SER56 PHE91 ASN92 TYR94 A: ASP147 HIS150 ARG152 THR153 PRO154 GLY196 HIS197 ASP198 GLU199 ASN200 TRP218 SER219 LYS220 LYS221 ILE222 ARG224 GLY244 SER245 ALA246 SER247 GLY248 ARG249 ALA250 ASP251 TRP295 LYS431 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
