Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2117 |
| PDBID: | 6CNJ |
| Chains: | GF_C |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/s41586-018-0081-7 |
| Antibody | |
| Antibody: | 12H2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Neuronal acetylcholine receptor subunit beta-2 |
| Antigen mutation: | No |
| Durg Target: | P17787; P17787; |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >6CNJ_G|Chain G, I, K|IgG1 Heavy Chain|Mus musculus (10090) MEWTWVFLFLLSVTAGVHSQVQLQQSGAEVMKPGASVKISCKGTGYTFSSYWIEWVKQRPGHGLERIGEILPGSGSTNYNEKFRGKATFTADKSSKTAYMQLSSLTSEDSAVYYCARYLPYYYAMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK |
Light Chain: F
Mutation: NULL
| >6CNJ_F|Chain F, H, J|IgG1 Kappa Light Chain|Mus musculus (10090) MKLPVRLLVLMFWIPASSSDVLMTQTPLSLPVSLGDQASISCRSSQSIVHSNGNTYLEWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSHVPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >6CNJ_C|Chain B, C, E|Neuronal acetylcholine receptor subunit beta-2|Homo sapiens (9606) TDTEERLVEHLLDPSRYNKLIRPATNGSELVTVQLMVSLAQLISVHEREQIMTTNVWLTQEWEDYRLTWKPEEFDNMKKVRLPSKHIWLPDVVLYNNADGMYEVSFYSNAVVSYDGSIFWLPPAIYKSACKIEVKHFPFDQQNCTMKFRSWTYDRTEIDLVLKSEVASLDDFTPSGEWDIVALPGRRNENPDDSTYVDITYDFIIRRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTVFLLLISKIVPPTSLDVPLVGKYLMFTMVLVTFSIVTSVCVLNVHHRSPTTHTMAPWVKVVFLEKLPALLFMQQPRHHDDDQERSVSEDWKYVAMVIDRLFLWIFVFVCVFGTIGMFLQPLFQNYTTTTFLHSDHSAPSSKSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: HIS31 SER32 VAL99 TRP101 G: THR29 SER31 SER32 TYR33 TRP34 LEU53 GLY55 SER56 GLY57 SER58 ASN60 LYS75 ARG99 LEU101 PRO102 TYR103 TYR104 TYR105 C: MET36 GLU133 LYS135 GLN141 ASN143 SER164 GLU165 VAL166 ALA167 SER168 LEU169 ASP170 PHE172 PRO174 ASP179 ILE180 VAL181 ALA182 LEU183 PRO184 GLY185 ASP202 ILE204 ARG206 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
