Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 211 |
| PDBID: | 2ADF |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.90 |
| Reference: | 10.1074/jbc.M508191200 |
| Antibody | |
| Antibody: | 82D6A3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Von Willebrand factor A3-domain |
| Antigen mutation: | No |
| Durg Target: | P04275 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >2ADF_H|Chain B[auth H]|82D6A3 IgG|Mus musculus (10090) QIQLVQSGPELKKPGETVKISCKASGYTFINYGMNWVKQAPGKGLKWMGWKNTNTGETTYGEEFRGRFAFSLETSVSTAYLQINNLKNEDTATYFCARDNPYYALDYWGQGTTVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Light Chain: L
Mutation: NULL
| >2ADF_L|Chain C[auth L]|82D6A3 IgG|Mus musculus (10090) DIQMTQSPSSLSASLGGKVTITCKASQDINKYIAWYQHKPGKGPRLLIHYTSTLQPGIPSRFSGSGSGRDYSFSISNLEPEDIATYYCLQYDNLRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFN |
Antigen
Chain: A
Mutation: NULL
| >2ADF_A|Chain A|Von Willebrand factor|Homo sapiens (9606) GSHMAPDCSQPLDVILLLDGSSSFPASYFDEMKSFAKAFISKANIGPRLTQVSVLQYGSITTIDVPWNVVPEKAHLLSLVDVMQREGGPSQIGDALGFAVRYLTSEMHGARPGASKAVVILVTDVSVDSVDAAADAARSNRVTVFPIGIGDRYDAAQLRILAGPAGDSNVVKLQRIEDLPTMVTLGNSFLHKLCSG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ILE2 THR28 ILE30 ASN31 TYR32 GLY33 TRP50 ASN52 ASN54 THR55 ARG98 ASP99 ASN100 PRO101 TYR102 TYR103 TYR107 L: LYS31 TYR32 HIS49 TYR50 THR53 GLN55 PRO56 TYR91 ASP92 LEU94 ARG95 A: SER974 ILE975 THR976 THR977 ILE978 ASP979 PRO981 TRP982 ASN983 VAL984 HIS990 LEU994 ASP1009 GLY1012 PHE1013 VAL1015 ARG1016 TYR1017 THR1019 MET1022 HIS1023 ALA1047 ALA1051 ASN1055 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
