Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2097 |
| PDBID: | 6C9W |
| Chains: | B_A |
| Organism: | Escherichia coli K-12, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1073/pnas.1801774115 |
| Antibody | |
| Antibody: | 9047 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | lactose permease of Escherichia coli (LacY) |
| Antigen mutation: | Yes |
| Durg Target: | P02920 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >6C9W_B|Chain B|Nanobody9047|Lama glama (9844) VQLVESGGGLVQAGDSLRLSCAASGGTFSTFNMGWFRQDLGKEREFVAAIRWTGGRAYYGDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCARQGTNGGGYSEATSYNYWGQGTQVTVSSH |
Antigen
Chain: A
Mutation: G46W/G262W
| >6C9W_A|Chain A|Lactose permease|Escherichia coli (strain K12) (83333) MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTWIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFWYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLY27 THR28 SER30 THR31 PHE32 ASN33 ARG52 TRP53 THR54 GLY55 ARG57 TYR59 ARG98 GLN99 GLY100 THR101 ASN102 GLY103 GLY104 GLY105 TYR106 SER107 GLU108 TYR114 A: HIS35 LYS42 GLN242 ASN245 THR248 SER249 PHE250 PHE251 ALA252 THR253 GLY254 GLU255 SER307 PHE308 ALA309 THR310 SER311 ALA312 TYR373 GLU374 SER375 ILE376 GLY377 PHE378 GLN379 GLY380 LEU383 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
