Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 2093 |
| PDBID: | 6C6Y |
| Chains: | HL_R |
| Organism: | Macaca mulatta, Betacoronavirus England 1 |
| Method: | XRD |
| Resolution (Å): | 3.32 |
| Reference: | 10.1128/JVI.02002-17 |
| Antibody | |
| Antibody: | JC57-14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | MERS Spike glycoprotein RBD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >6C6Y_H|Chain A, C[auth H]|JC57-14 Heavy chain|Macaca mulatta (9544) QVQLQESGPGLVKPSETLSLTCAVSGDSISSNYWSWIRQPPGKGLEWIGRFSGSGGSTDFNPSLKSRVTISTDTSKNQFSLNLRSVTAADTAVYYCAKTYSGTFDYWGQGVLVTVSSASTKGPSVFPLAPSSRSTSESTAALGCLVKDYFPEPVTVSWNSGSLTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYVCNVNHKPSNTKVDKRVEIA |
Light Chain: L
Mutation: NULL
| >6C6Y_L|Chain B, D[auth L]|JC57-14 Light chain|Macaca mulatta (9544) DIQMTQSPSSLSASVGDRVTITCRASQDINNYLSWYQQKPGKAPKPLIYYASSLETGVPSRFSGSRSGTDYTLTISSLQLEDFATYYCQQYNNSPYSFGQGTKVEIKRTVAAPSVFIFPPSEDQVKSGTVSVVCLLNNFYPREASVKWKVDGALKTGNSQESVTEQDSKDNTYSLSSTLTLSSTEYQSHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: R
Mutation: NULL
| >6C6Y_R|Chain E[auth R], F[auth S]|Spike glycoprotein|Betacoronavirus England 1 (1263720) VECDFSPLLSGTPPQVYNFKRLVFTNCNYNLTKLLSLFSVNDFTCSQISPAAIASNCYSSLILDYFSYPLSMKSDLSVSSAGPISQFNYKQSFSNPTCLILATVPHNLTTITKPLKYSYINKCSRFLSDDRTEVPQLVNANQYSPCVSIVPSTVWEDGDYYRKQLSPLEGGGWLVASGSTVAMTEQLQMGFGITVQYGTDTNSVCPKL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLN1 SER31 ASN32 TYR33 ARG50 SER56 LYS94 THR95 TYR96 SER97 GLY98 ASP101 TYR102 L: GLN27 ASP28 ASN30 TYR32 TYR49 TYR50 SER53 LEU54 GLU55 THR56 GLY57 VAL58 TYR91 ASN92 TYR96 R: GLY391 PRO394 TYR397 ASN398 PHE399 LYS400 TYR523 VAL527 SER528 ILE529 VAL530 PRO531 SER532 TRP535 GLU536 ASP539 TYR540 TYR541 ARG542 LYS543 GLN544 LEU545 SER546 PRO547 LEU548 GLU549 TRP553 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
